Cryptic kanamycin resistance gene in Streptomyces griseus.
نویسندگان
چکیده
منابع مشابه
Cloning of the kanamycin resistance gene from a kanamycin-producing Streptomyces species.
A kanamycin-producing strain, Streptomyces kanamyceticus ISP5500, is resistant to kanamycin. A kanamycin resistance determinant was cloned from S. kanamyceticus into Streptomyces lividans 1326, using the plasmid vector pIJ702. The resulting plasmid, pMCP5, could also transform Streptomyces lavendulae S985 and Streptomyces parvulus 2283 to kanamycin resistance. Transformants carrying pMCP5 were ...
متن کاملSelf-cloning in Streptomyces griseus of an str gene cluster for streptomycin biosynthesis and streptomycin resistance.
An str gene cluster containing at least four genes (strR, strA, strB, and strC) involved in streptomycin biosynthesis or streptomycin resistance or both was self-cloned in Streptomyces griseus by using plasmid pOA154. The strA gene was verified to encode streptomycin 6-phosphotransferase, a streptomycin resistance factor in S. griseus, by examining the gene product expressed in Escherichia coli...
متن کاملGenetic recombination in Streptomyces griseus.
Low-frequency (10(-6)) genetic recombination was observed in a cephamycin-producing strain of Streptomyces griseus. The recombinants were predominantly heteroclones. Heteroclone analysis was performed involving four heteroclones of one cross. In 100 mutants correlation was found between the type of auxotrophy and the level of antibiotic activity. A cross of this strain with a streptomycin-produ...
متن کاملOxidation of Meloxicam by Streptomyces griseus
The aim of the present investigation was to biotransform the anti-inflammatory compound meloxicam by enzymes present in whole cells of five actinomycete cultures to produce novel bioactive derivatives. Among the actinomycetes screened, Streptomyces griseus NCIM 2622 was found to possess the enzyme system(s) that oxidize meloxicam into two metabolites whereas that present in S. griseus NCIM 2623...
متن کاملThe kanamycin biosynthetic gene cluster from Streptomyces kanamyceticus.
Kanamycin is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus1). Although it possesses broad range antibacterial activity, bacteria resistant to this antibiotic readily appear, and therefore its clinical use has been restricted to tuberculosis. Resistant bacteria inactivate kanamycin by modifying its specific aminoor hydroxygroup using acetyltransferase, phosphotransferase an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Actinomycetologica
سال: 1990
ISSN: 1881-6371,0914-5818
DOI: 10.3209/saj.4_33